9HTO

Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with glutarimide based compound 2r

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-16
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.8856
Spacegroup name	P 21 21 21
Unit cell lengths	56.052, 59.905, 88.518
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.950 - 1.850
R-factor	0.19849
Rwork	0.196
R-free	0.24006
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.012
RMSD bond angle	1.758
Data reduction software	XDS
Refinement software	REFMAC (5.8.0266)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.960
High resolution limit [Å]	1.850	1.850
Number of reflections	26222	4091
<I/σ(I)>	12.01	1.39
Completeness [%]	99.7
Redundancy	13.01
CC(1/2)	0.997	0.616

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.4-0.6 M (NH4)H2PO4, ~17 mg/ml MSCI4 with 3mM thalidomide