9HTL

Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with glutarimide based compound 2j

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-11
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	56.507, 59.361, 88.337
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.650 - 1.740
R-factor	0.18549
Rwork	0.184
R-free	0.21710
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.009
RMSD bond angle	1.573
Data reduction software	XDS
Refinement software	REFMAC (5.8.0266)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.850
High resolution limit [Å]	1.740	1.740
Number of reflections	58605	9397
<I/σ(I)>	14.31	1.57
Completeness [%]	99.8	99.5
Redundancy	6.96	6.79
CC(1/2)	0.999	0.611

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.4-0.6 M (NH4)H2PO4, ~17 mg/ml MSCI4 with 3 mM thalidomide