Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-07 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 112.158, 112.158, 99.312 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.970 - 1.500 |
| R-factor | 0.1769 |
| Rwork | 0.176 |
| R-free | 0.19880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.589 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.970 | 1.520 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.139 | 1.582 |
| Rmeas | 0.142 | 1.621 |
| Rpim | 0.027 | 0.347 |
| Number of reflections | 102148 | 5025 |
| <I/σ(I)> | 17.4 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 26.7 | 21 |
| CC(1/2) | 0.999 | 0.613 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 20% PEG 3350, 0.2 M zinc acetate, 0.1 M Imidazole pH 7.8 |
