Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-11 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 63 |
| Unit cell lengths | 46.078, 46.078, 139.565 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 69.780 - 1.070 |
| R-factor | 0.1694 |
| Rwork | 0.169 |
| R-free | 0.18370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.338 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.780 | 1.090 |
| High resolution limit [Å] | 1.070 | 1.070 |
| Rmerge | 0.108 | 1.176 |
| Rmeas | 0.111 | 1.471 |
| Rpim | 0.025 | 0.863 |
| Number of reflections | 71473 | 2731 |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 97.8 | 75.9 |
| Redundancy | 16 | 2.5 |
| CC(1/2) | 0.999 | 0.334 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 18% PEG 6000, 1 M lithium chloride, 0.1 M tri-Sodium citrate pH 4.0 |
