Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-11 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 86.307, 86.307, 72.738 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.130 - 1.880 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.22410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.113 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.130 | 1.910 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.108 | 1.550 |
| Rmeas | 0.111 | 1.595 |
| Rpim | 0.027 | 0.374 |
| Number of reflections | 25883 | 1268 |
| <I/σ(I)> | 16.6 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 17.3 | 18.2 |
| CC(1/2) | 0.998 | 0.849 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.6 M magnesium sulfate, 0.1 M MES pH 6.5 |
