9HRI
Human holo aromatic L-amino acid decarboxylase (AADC) L353P variant native structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 107.246, 107.246, 219.192 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.170 - 2.050 |
| R-factor | 0.1899 |
| Rwork | 0.189 |
| R-free | 0.20950 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.631 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | FFT |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.170 | 2.110 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.063 | 0.530 |
| Number of reflections | 47570 | 3632 |
| <I/σ(I)> | 24.6 | 4.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.6 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.15 | 0.1 M Hepes, 40% PEG 200 |
