9HR1
Crystal structure of GH19 domain of D29-LysA (Form II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.606, 89.052, 113.391 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.040 - 2.000 |
| R-factor | 0.1695 |
| Rwork | 0.168 |
| R-free | 0.20450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.735 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 113.390 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.117 | 0.895 |
| Number of reflections | 30434 | 2255 |
| <I/σ(I)> | 17.2 | |
| Completeness [%] | 96.9 | 100 |
| Redundancy | 14.3 | 15 |
| CC(1/2) | 0.999 | 0.880 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 12% (v/v) ethylene glycol; 6 % (w/v) PEG 8K, 0.1 M imidazole/MES buffer pH 6.5, and DL-amino-acids (glutamic acid, alanine, glycine; lysine, serine) at 20 mM |
