9HP7
Crystal structure of GH19 E228Q domain of D29-LysA in complex with (GlcNAc)4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.062, 44.845, 69.530 |
| Unit cell angles | 90.00, 126.42, 90.00 |
Refinement procedure
| Resolution | 38.250 - 1.830 |
| R-factor | 0.1811 |
| Rwork | 0.180 |
| R-free | 0.20070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.659 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.250 | 1.858 |
| High resolution limit [Å] | 1.826 | 1.826 |
| Rmerge | 0.073 | 0.758 |
| Number of reflections | 19241 | 991 |
| <I/σ(I)> | 15.1 | |
| Completeness [%] | 95.1 | 99.9 |
| Redundancy | 6.6 | 5.7 |
| CC(1/2) | 0.999 | 0.872 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Condition E1 from Morpheus crystal screening: - 0.12M Ethylene glycols - 0.1M Buffer System 1 pH 6.5 - 30% Precipitant mix 1 |
