9HNL
Structure of the (6-4) photolyase of Caulobacter crescentus in its oxidized state at room temperture-synchrotron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2020-03-26 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.347, 102.677, 103.539 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.990 - 2.200 |
| R-factor | 0.1953 |
| Rwork | 0.193 |
| R-free | 0.23750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.530 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.990 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.200 | 1.065 |
| Number of reflections | 27390 | 2689 |
| <I/σ(I)> | 7.5 | 1.89 |
| Completeness [%] | 99.6 | |
| Redundancy | 4 | |
| CC(1/2) | 0.996 | 0.524 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 8.5 | 293 | 0.1 M Tris (pH 8.5) 0.2 M MgCl2 20% (w/v) PEG3350 |
