9HNH
A7 Synthetic Alpha solenoid Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.61995 |
| Spacegroup name | I 41 |
| Unit cell lengths | 74.841, 74.841, 50.878 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.960 - 2.830 |
| R-factor | 0.2587 |
| Rwork | 0.257 |
| R-free | 0.30080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.445 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.960 | 2.880 |
| High resolution limit [Å] | 2.830 | 2.830 |
| Rmerge | 0.173 | 2.170 |
| Rmeas | 0.189 | 2.434 |
| Rpim | 0.074 | 1.053 |
| Number of reflections | 3399 | 149 |
| <I/σ(I)> | 7.5 | 0.8 |
| Completeness [%] | 99.6 | 92.5 |
| Redundancy | 3.4 | 2.7 |
| CC(1/2) | 0.989 | 0.628 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 25% (w/v) PEG 1500, 100 mM PCB buffer pH 7.0 |
