9HNG

Structure of A16/G9 (G9 mutant - H44Y) of Vaccinia virus in complex with VHH D07

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-03
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980112
Spacegroup name	P 21 21 21
Unit cell lengths	46.102, 147.467, 183.367
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.090 - 2.500
R-factor	0.217
Rwork	0.215
R-free	0.24710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.381
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.670	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	44387	4554
<I/σ(I)>	12.8
Completeness [%]	99.9
Redundancy	9.7
CC(1/2)	0.999	0.450

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	20% (w/v) PEG 4K, 10% (v/v) glycerol, 0.2M MgSO4 (cryoprotected in 20% glycerol)