9HNC

Crystal structure of potassium-independent L-asparaginase from Phaseolus vulgaris (PvAIII, PvAspG2)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.729310
Spacegroup name	P 1 2 1
Unit cell lengths	123.764, 123.645, 187.679
Unit cell angles	90.00, 90.06, 90.00

Refinement procedure

Resolution	47.688 - 1.879
Rwork	0.182
R-free	0.21110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.717
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.690	1.990
High resolution limit [Å]	1.879	1.879
Rmerge	0.083	0.813
Rmeas	0.074	0.774
Number of reflections	451021	73624
<I/σ(I)>	13.43	2.1
Completeness [%]	98.7
Redundancy	6.93
CC(1/2)	0.998	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2.0 M ammonium sulfate and 5% v/v 2-propanol