9HMZ
Structure of Corynebacterium glutamicum SteA, a cell division regulator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-16 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 126.506, 128.720, 211.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 105.920 - 2.050 |
| R-factor | 0.2068 |
| Rwork | 0.205 |
| R-free | 0.23820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.900 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 105.925 | 2.333 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 126836 | 6342 |
| <I/σ(I)> | 9.2 | 1.8 |
| Completeness [%] | 95.6 | 73.9 |
| Redundancy | 13.6 | 12.3 |
| CC(1/2) | 0.992 | 0.703 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 10 %w/v PEG 8K 0.1 M Imidazole 8 pH 0.2 M Calcium Acetate |
