9HMU
DUF4465 domain containing protein in complex with vitamin B12.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 77.36 |
| Detector technology | PIXEL |
| Collection date | 2024-11-22 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9655 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 113.720, 113.720, 162.593 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 84.379 - 1.850 |
| Rwork | 0.190 |
| R-free | 0.21320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.198 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.88)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.379 | 1.879 |
| High resolution limit [Å] | 1.800 | 1.849 |
| Rmerge | 0.131 | 1.362 |
| Rmeas | 0.147 | 0.751 |
| Rpim | 0.091 | 0.964 |
| Number of reflections | 96344 | 20050 |
| <I/σ(I)> | 9.3 | 1 |
| Completeness [%] | 92.1 | 96.8 |
| Redundancy | 12.8 | 1.299 |
| CC(1/2) | 0.907 | 0.373 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298.15 | 35% (poly acrylic acid sodium salt) 2100 0.1M HEPES (pH 7.5) 0.2M ammonium sulfate |
