9HMM

Crystal structure of mouse ADAT2/ADAT3 tRNA deamination complex A180V mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00001
Spacegroup name	P 2 21 21
Unit cell lengths	51.903, 106.398, 129.199
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.190 - 2.900
R-factor	0.1978
Rwork	0.195
R-free	0.24480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.129
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19rc4_4035)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.080
High resolution limit [Å]	2.900	2.900
Rmerge	0.036	0.303
Number of reflections	16404	2594
<I/σ(I)>	8.13	1.12
Completeness [%]	99.7
Redundancy	7.8
CC(1/2)	0.991	0.383

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	18% (W/V) PEG3350, 0.1 M BIS-TRIS PROPANE PH 7.5, 0.2 M NABR