9HLL

murine aM I-domain in complex with nanobody aCR3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-17
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.9762
Spacegroup name	C 2 2 21
Unit cell lengths	78.402, 127.444, 125.855
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.040 - 2.170
R-factor	0.2057
Rwork	0.202
R-free	0.25940
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.222
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.040	2.230
High resolution limit [Å]	2.170	2.170
Rmerge	0.071	0.881
Number of reflections	33520	2314
<I/σ(I)>	20.64
Completeness [%]	99.6	97.2
Redundancy	13.6	13.7
CC(1/2)	1.000	0.969

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	PEG pH 7.0