9HLJ
Crystal structure of GV37-TCR in complex with HLA-C*12:02 with KAYNVTQAF (KF9), a 9-mer epitope from SARS-CoV-2 Nucleocapsid (N266-274)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-10-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.953732 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.006, 59.468, 109.847 |
| Unit cell angles | 90.00, 109.91, 90.00 |
Refinement procedure
| Resolution | 45.460 - 2.540 |
| R-factor | 0.2129 |
| Rwork | 0.211 |
| R-free | 0.25740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.443 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.460 | 2.660 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Number of reflections | 29890 | 3299 |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 98.6 | 89.2 |
| Redundancy | 6.8 | 6.9 |
| CC(1/2) | 0.995 | 0.691 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Magnesium formate dihydrate, 20% PEG3350 |
