9HKC
Crystal structure of IMPDH from Burkholderia thailandensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.978565 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.540, 72.560, 93.230 |
| Unit cell angles | 90.00, 124.73, 90.00 |
Refinement procedure
| Resolution | 46.180 - 1.900 |
| R-factor | 0.1856 |
| Rwork | 0.184 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.835 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.180 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 59054 | 5810 |
| <I/σ(I)> | 18.04 | |
| Completeness [%] | 99.8 | |
| Redundancy | 7 | |
| CC(1/2) | 1.000 | 0.705 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291.15 | 5%w/v PEG 4K, 30%w/v MPD, 0.1M HEPES 7.5 pH |
