9HK7
Crystal structure of IMPDH from Burkholderia thailandensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.978565 |
| Spacegroup name | I 4 |
| Unit cell lengths | 119.600, 119.600, 143.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.950 - 1.780 |
| R-factor | 0.1787 |
| Rwork | 0.173 |
| R-free | 0.18480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.608 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.950 | 1.845 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Number of reflections | 95888 | 9544 |
| <I/σ(I)> | 10.57 | |
| Completeness [%] | 99.8 | |
| Redundancy | 13.6 | |
| CC(1/2) | 0.996 | 0.766 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 20%w/v PEG 3350; 0.2 M LiNO3 |
