9HJO
FANCM-FAAP24-dsDNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.06 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.008, 69.975, 86.760 |
| Unit cell angles | 91.63, 89.93, 115.95 |
Refinement procedure
| Resolution | 86.718 - 2.401 |
| Rwork | 0.214 |
| R-free | 0.26450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.726 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.88)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.720 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rpim | 0.054 | 0.928 |
| Number of reflections | 48487 | 1780 |
| <I/σ(I)> | 17 | 1.1 |
| Completeness [%] | 96.2 | 70.19 |
| Redundancy | 3.9 | |
| CC(1/2) | 0.998 | 0.344 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25% PEG3350 0.1M di-ammonium monohydrogen phosphate |
