9HJ7
Structure of Chitinase 35 from Metschnikowia pulcherrima (MpChit35)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.582, 54.850, 97.776 |
| Unit cell angles | 90.00, 107.72, 90.00 |
Refinement procedure
| Resolution | 47.310 - 2.550 |
| R-factor | 0.18386 |
| Rwork | 0.181 |
| R-free | 0.23817 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.436 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.310 | 2.660 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.184 | 0.474 |
| Rmeas | 0.201 | 0.521 |
| Rpim | 0.080 | 0.213 |
| Number of reflections | 20240 | 2416 |
| <I/σ(I)> | 7.5 | 4.7 |
| Completeness [%] | 99.5 | 97.4 |
| Redundancy | 6.1 | 5.8 |
| CC(1/2) | 0.985 | 0.915 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 22% PEG 4000, 100mM magnesium chloride, 100mM sodium acetate pH 4.6 |
