9HIH

Mouse IL-2 fused to S4B6 Fab fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-21
Detector	DECTRIS EIGER2 X CdTe 16M
Wavelength(s)	0.976254
Spacegroup name	P 21 21 21
Unit cell lengths	42.866, 77.004, 169.659
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	77.000 - 2.700
R-factor	0.23754
Rwork	0.235
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless (0.7.15)
Phasing software	SIMBAD (0.2.8)
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.000	2.830
High resolution limit [Å]	2.700	2.700
Number of reflections	15862	2095
<I/σ(I)>	2.7
Completeness [%]	98.8
Redundancy	7.6
CC(1/2)	0.891	0.052

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	20% w/v PEG MME 2000 0.2M TMAO 0.1M Tris, pH 8.5