9HHX

Plant membrane receptor IGP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033286
Spacegroup name	P 61
Unit cell lengths	84.365, 84.365, 198.320
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.068 - 2.620
Rwork	0.182
R-free	0.25640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	2.335
Data scaling software	pointless (1.13.4)
Phasing software	PHENIX (2.8.3)
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.88))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.580	49.580	2.740
High resolution limit [Å]	2.620	9.080	2.620
Rmerge	0.428	0.070	2.114
Rmeas	0.475	0.078	2.412
Rpim	0.205	0.033	1.147
Number of reflections	24021	601	2935
<I/σ(I)>	5.9	27.1	1
Completeness [%]	100.0	99.5	100
Redundancy	10.5	10.6	8.7
CC(1/2)	0.973	0.998	0.314

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	292.15	0.1 M Sodium acetate trihydrate pH 4.6 0.2 M Ammonium sulfate 30 % PEG 2000