9HHU

Plant membrane receptor IGP1 in complex with cellotriose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033286
Spacegroup name	P 61
Unit cell lengths	83.775, 83.775, 198.935
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.950 - 2.160
Rwork	0.169
R-free	0.21050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	2.289
Data scaling software	pointless (1.13.4)
Phasing software	PHENIX (2.8.3)
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.88))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.950	48.950	2.230
High resolution limit [Å]	2.160	8.910	2.160
Rmerge	0.120	0.036	2.067
Rmeas	0.138	0.041	2.474
Rpim	0.069	0.020	1.336
Number of reflections	42267	630	3633
<I/σ(I)>	10	35.1	1
Completeness [%]	100.0	99.1	100
Redundancy	7.8	7.8	6.5
CC(1/2)	0.998	0.999	0.316

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	292.15	100 mM Sodium acetate pH 4.6, 200 mM Ammonium sulfate, 30 % w/v PEG 2000