9HH7

Crystal Structure of the Plasmodium falciparum Bromodomain PfBDP1 in complex with RMM23

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-15
Detector	DECTRIS EIGER2 X CdTe 16M
Wavelength(s)	0.88560
Spacegroup name	I 2 2 2
Unit cell lengths	38.120, 88.215, 108.493
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.960 - 1.790
R-factor	0.2105
Rwork	0.209
R-free	0.24320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.393
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.965	1.944
High resolution limit [Å]	1.787	1.787
Rmerge	0.070
Rmeas	0.076
Number of reflections	9402	1146
<I/σ(I)>	13.4
Completeness [%]	91.8
Redundancy	6.3
CC(1/2)	0.999	0.612

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	20% PEG MMW (PEG 2K, 3350, 4K and 5K MME ;each 12.5 %) ,7% (v/v) Tacsimate, 0.1 M HEPES pH 7.2