9HH3
Structure of the apo ZgCgsA carrageenan-sulfatase (S1_19) from the marine bacterium Zobellia galactanivorans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-04-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.978565 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.800, 199.150, 75.350 |
| Unit cell angles | 90.00, 99.33, 90.00 |
Refinement procedure
| Resolution | 49.360 - 2.260 |
| R-factor | 0.1975 |
| Rwork | 0.195 |
| R-free | 0.24590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.947 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.360 | 2.345 |
| High resolution limit [Å] | 2.260 | 2.264 |
| Rmerge | 0.108 | 1.321 |
| Rmeas | 0.116 | |
| Rpim | 0.042 | 0.518 |
| Number of reflections | 99086 | 9564 |
| <I/σ(I)> | 13.37 | |
| Completeness [%] | 99.6 | 96.66 |
| Redundancy | 7.7 | 7.4 |
| CC(1/2) | 0.998 | 0.564 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 0.1 M Bis-Tris Propane pH 7.5, 0.2 M NaBr, 10 % PEG 3350 |
