9HGD
Crystal structure of human GABARAP in complex with cyclic peptide GAB_D23
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM07 |
| Synchrotron site | ESRF |
| Beamline | BM07 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.980 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.630, 43.790, 67.520 |
| Unit cell angles | 90.00, 128.38, 90.00 |
Refinement procedure
| Resolution | 18.420 - 1.500 |
| R-factor | 0.1896 |
| Rwork | 0.188 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.836 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.020 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmeas | 0.067 | 3.389 |
| Number of reflections | 39046 | 2844 |
| <I/σ(I)> | 13.34 | 0.58 |
| Completeness [%] | 97.0 | 95.5 |
| Redundancy | 6.85 | 6.62 |
| CC(1/2) | 0.999 | 0.346 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.1 M MES pH 5, 30% PEG 6000 |
