9HGC
Crystal structure of human GABARAPL1 in complex with cyclic peptide GAB_D8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM07 |
| Synchrotron site | ESRF |
| Beamline | BM07 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.290, 116.810, 116.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.420 - 2.520 |
| R-factor | 0.2337 |
| Rwork | 0.215 |
| R-free | 0.25860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.410 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MoRDa |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.420 | 2.590 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmeas | 0.238 | 3.279 |
| Number of reflections | 24336 | 1803 |
| <I/σ(I)> | 10.01 | 0.73 |
| Completeness [%] | 99.4 | 99.8 |
| Redundancy | 13.3 | 13.8 |
| CC(1/2) | 0.996 | 0.291 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.17 M ammonium sulfate, 25.5% PEG 4000, 15% glycerol |
