9HEF
Crystal Structure of MfpD from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 291 |
| Detector technology | PIXEL |
| Collection date | 2019-05-07 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.735, 73.650, 49.269 |
| Unit cell angles | 90.00, 111.39, 90.00 |
Refinement procedure
| Resolution | 45.880 - 1.830 |
| Rwork | 0.232 |
| R-free | 0.26960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 1.920 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Number of reflections | 35875 | 3487 |
| <I/σ(I)> | 14.13 | |
| Completeness [%] | 99.3 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.990 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20 mM Na Acetate, 60 % MPD |
