9HC3
Apo-state structure of the human metabotropic glutamate receptor 5 transmembrane domain freeze-trapped after light activation of photoswitchable ligand alloswitch-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-03 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.221, 43.363, 82.642 |
| Unit cell angles | 90.00, 99.86, 90.00 |
Refinement procedure
| Resolution | 19.930 - 2.900 |
| R-factor | 0.2853 |
| Rwork | 0.284 |
| R-free | 0.30180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.466 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.400 | 2.810 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.092 | 0.973 |
| Number of reflections | 9587 | 603 |
| <I/σ(I)> | 8.5 | 1.2 |
| Completeness [%] | 68.9 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.998 | 0.474 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 7.2 | 293 | PEG300, di-ammonium hydrogen phosphate, 1,6-hexanediol, BIS-TRIS |
