9HC2
Crystal structure of Lysozyme in complex with Gentisic Acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-07 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.974, 77.974, 37.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.140 - 1.270 |
| R-factor | 0.1929 |
| Rwork | 0.192 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.984 |
| Data reduction software | XDS (1.20.1_4487) |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.140 | 1.290 |
| High resolution limit [Å] | 1.270 | 1.270 |
| Rmerge | 0.089 | 3.111 |
| Rmeas | 0.091 | 3.348 |
| Rpim | 0.019 | 1.193 |
| Number of reflections | 30172 | 1266 |
| <I/σ(I)> | 15.6 | 0.5 |
| Completeness [%] | 98.1 | 84.3 |
| Redundancy | 21.8 | 7.3 |
| CC(1/2) | 1.000 | 0.325 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 100 mM tri-Sodium citrate pH 4.0, 800 mM Ammonium sulfate |
