9HB2

Structure of the truncated version of IdeC protease C94S from Streptococcus canis

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 3
Unit cell lengths	109.379, 109.379, 109.379
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.960 - 2.250
R-factor	0.18004
Rwork	0.178
R-free	0.21374
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.606
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.960	2.320
High resolution limit [Å]	2.250	2.250
Rpim	0.059	0.706
Number of reflections	21011	1916
<I/σ(I)>	11.3	1.2
Completeness [%]	100.0	100
Redundancy	12.6	12.7
CC(1/2)	0.997	0.431

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	20% PEG 3350, 200 mM Na/K phosphate, and 100 mM Bis Tris propane pH 6.5