9HB1
Structure of IdeC protease C94S from Streptococcus canis
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 70.253, 70.253, 94.884 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.440 - 2.700 |
| Rwork | 0.202 |
| R-free | 0.26750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.440 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rpim | 0.138 | 0.792 |
| Number of reflections | 7819 | 1006 |
| <I/σ(I)> | 4.8 | 1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.5 | 9.5 |
| CC(1/2) | 0.977 | 0.447 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 30% PEG 3350, 200 mM MgCl2 and 100 mM Tris HCl pH 8.5 |
