9H9V
BabyBass soluble domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-17 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.041, 119.621, 64.172 |
| Unit cell angles | 90.00, 92.52, 90.00 |
Refinement procedure
| Resolution | 21.240 - 2.420 |
| R-factor | 0.2674 |
| Rwork | 0.264 |
| R-free | 0.32740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.930 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.520 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.062 | 0.413 |
| Number of reflections | 30506 | 3193 |
| <I/σ(I)> | 10.13 | |
| Completeness [%] | 98.1 | |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 30% (w/v) PEG 8,000, 100 mM sodium acetate pH 4.5, 200 mM lithium sulfate |
