9H7T
MITF in complex with 6-((1-hydroxycyclohexyl)ethynyl)-4-methyl-1-phenyl-1H-indole-2-carboxylic acid
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000290 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 90.419, 90.419, 82.785 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.600 - 1.850 |
| R-factor | 0.2075 |
| Rwork | 0.206 |
| R-free | 0.23790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.092 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.059 | 1.878 |
| High resolution limit [Å] | 1.846 | 1.846 |
| Rpim | 0.010 | 0.332 |
| Number of reflections | 30094 | 1476 |
| <I/σ(I)> | 35.4 | 2.2 |
| Completeness [%] | 100.0 | |
| Redundancy | 20 | |
| CC(1/2) | 1.000 | 0.833 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.2 M ammonium sulfate, 5 % (v/v) PEG 400, 0.1 M MES pH 6.5 |
