9H6W
Crystal structure of the Salmonella effector SspH1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I23 |
| Synchrotron site | Diamond |
| Beamline | I23 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.62 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 170.772, 170.772, 94.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.310 - 2.900 |
| R-factor | 0.262 |
| Rwork | 0.260 |
| R-free | 0.31580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.352 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.310 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.499 | |
| Rmeas | 0.506 | |
| Rpim | 0.080 | |
| Number of reflections | 18583 | 73343 |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 39.6 | |
| CC(1/2) | 0.996 | 0.416 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 0.225 M sodium tartrate, 22% PEG 3350, 11 mM sarcosine |
