9H6V
Lotus japonicus CERK6 extracellular domain in complex with two nanobodies
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-10-10 |
| Detector | DECTRIS EIGER2 XE CdTe 16M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.280, 57.580, 86.810 |
| Unit cell angles | 96.97, 88.98, 96.42 |
Refinement procedure
| Resolution | 32.420 - 2.190 |
| R-factor | 0.2068 |
| Rwork | 0.204 |
| R-free | 0.25240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.487 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.971 | 2.506 |
| High resolution limit [Å] | 2.186 | 2.186 |
| Rmerge | 0.226 | 1.550 |
| Rmeas | 0.244 | 1.684 |
| Rpim | 0.092 | 0.647 |
| Number of reflections | 25361 | 1269 |
| <I/σ(I)> | 8.2 | 1.3 |
| Completeness [%] | 52.0 | 7.7 |
| Redundancy | 7 | 6.5 |
| CC(1/2) | 0.993 | 0.187 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 292.15 | 0.2 M sodium bromide 20 % w/v PEG 3350 |
