9H68

Crystal Structure of the spore gernation lytic transglycosylase SleC from Clostridioides difficile in its zymogenic form (prepro-SleC)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-15
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97926
Spacegroup name	P 1
Unit cell lengths	66.431, 89.781, 99.621
Unit cell angles	76.90, 75.87, 89.12

Refinement procedure

Resolution	72.900 - 2.100
R-factor	0.2137
Rwork	0.211
R-free	0.26110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.023
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	96.030	2.360
High resolution limit [Å]	2.100	2.100
Number of reflections	72598	3454
<I/σ(I)>	8.7
Completeness [%]	98.4
Redundancy	5.3
CC(1/2)	0.990	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M sodium citrate (pH 5.6) and 10% Jeffamine M-2000