9H5F

MITF(217-296)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00002
Spacegroup name	P 41 21 2
Unit cell lengths	90.640, 90.640, 82.773
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.090 - 2.300
R-factor	0.2318
Rwork	0.231
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.289
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.092	2.340
High resolution limit [Å]	2.300	2.300
Rpim	0.026	0.244
Number of reflections	15627	749
<I/σ(I)>	18.7
Completeness [%]	98.5
Redundancy	13
CC(1/2)	0.999	0.874

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2.2 M ammonium sulfate, 5 % (v/v) PEG 400, 0.1 M MES pH 6.5