9H45

Crystal Structure of Hfq V22A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-23
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	C 1 2 1
Unit cell lengths	116.250, 67.150, 84.580
Unit cell angles	90.00, 104.64, 90.00

Refinement procedure

Resolution	56.240 - 2.080
R-factor	0.2764
Rwork	0.274
R-free	0.32330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.679
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.660	2.120
High resolution limit [Å]	2.080	2.080
Rmerge	0.509	2.025
Rmeas	0.551	2.196
Rpim	0.209	0.841
Total number of observations	258949	12714
Number of reflections	37712	1912
<I/σ(I)>	3.3	1.5
Completeness [%]	99.1
Redundancy	6.9	6.6
CC(1/2)	0.968	0.246

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	15 %v/v PEGSH 0.15 M NH4 Acet 0.1 M Na3 Cit