9H41

Apo-Helical Carotenoid Protein 4 (HCP4) from Anabaena G152C mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9655
Spacegroup name	P 43 21 2
Unit cell lengths	60.871, 60.871, 112.389
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.970 - 3.090
R-factor	0.2039
Rwork	0.202
R-free	0.24250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.161
Data reduction software	XDS
Data scaling software	pointless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9.970	3.340
High resolution limit [Å]	3.090	3.090
Rmerge	0.140
Number of reflections	40807	4175
<I/σ(I)>	11.9
Completeness [%]	96.1
Redundancy	10.1
CC(1/2)	0.994	0.943

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.08M Tris pH 8.5, 1.6M Ammonium dihydrogen phosphate, 20% v/v Glycerol