9H40

Pinoresinol hydroxylase from Pseudomonas sp.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.965460
Spacegroup name	P 21 21 21
Unit cell lengths	56.214, 131.809, 137.458
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.760 - 1.800
R-factor	0.18849
Rwork	0.186
R-free	0.22772
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	REFMAC (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.430	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.060
Number of reflections	93635	4624
<I/σ(I)>	12.6
Completeness [%]	98.7
Redundancy	4.1
CC(1/2)	0.998	0.389

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Morpheus H5