9H3E
Hen egg white lysozyme crystallization and structure determination at room temperature in the CrystalChip
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM07 |
| Synchrotron site | ESRF |
| Beamline | BM07 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2024-07-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.160, 79.160, 38.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.400 - 1.500 |
| R-factor | 0.1398 |
| Rwork | 0.138 |
| R-free | 0.17700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.713 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.400 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.220 | |
| Rmeas | 0.234 | |
| Number of reflections | 37004 | 21510 |
| <I/σ(I)> | 9.49 | 0.94 |
| Completeness [%] | 99.6 | 97.9 |
| Redundancy | 9.92 | 7.88 |
| CC(1/2) | 0.997 | 0.395 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTER-DIFFUSION | 4.5 | 293 | Protein crystallized by counter-diffusion in the CrystalChip, stock solution 60 mg/ml in 50 mM Na acetate pH 4.5, reservoir solution 2 M NaCl, 50 mM Na acetate pH 4.5 |
