9H3D

F-ENA exosporium anchoring complex between ExsF and a peptide derived from the N-terminus of F-Anchor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-24
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97856
Spacegroup name	P 1 21 1
Unit cell lengths	51.292, 91.809, 99.931
Unit cell angles	90.00, 101.57, 90.00

Refinement procedure

Resolution	50.250 - 1.920
R-factor	0.1859
Rwork	0.184
R-free	0.21580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.847
Data reduction software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.250	10.000
High resolution limit [Å]	1.920	1.920
Rpim	0.103	0.969
Number of reflections	69027	3362
<I/σ(I)>	5.9
Completeness [%]	100.0
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	6% w/v PEG3350, 0.15 M Sodium Chloride and 4M Potassium iodide