9H38
C-terminal domain of the F-ENA tip fibrillum F-BclA from Bacillus thuringiensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-30 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.826, 113.486, 113.599 |
| Unit cell angles | 60.11, 89.19, 86.36 |
Refinement procedure
| Resolution | 49.240 - 2.290 |
| R-factor | 0.1882 |
| Rwork | 0.185 |
| R-free | 0.24180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.861 |
| Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.240 | 2.292 |
| High resolution limit [Å] | 2.290 | 2.292 |
| Rmerge | 0.148 | |
| Rmeas | 0.617 | |
| Rpim | 0.423 | |
| Number of reflections | 128674 | 8218 |
| <I/σ(I)> | 4.9 | |
| Completeness [%] | 97.8 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 6% w/v PEG3350, 0.15 M Sodium Chloride and 4M Potassium iodide |
