9H36

Crystal structure of the YTHDC2 YTH domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97625
Spacegroup name	P 62 2 2
Unit cell lengths	117.681, 117.681, 103.876
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.750 - 2.620
R-factor	0.2475
Rwork	0.247
R-free	0.25170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.146
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	101.915	2.665
High resolution limit [Å]	2.620	2.620
Number of reflections	13260	636
<I/σ(I)>	36.8
Completeness [%]	100.0
Redundancy	38.8
CC(1/2)	0.998	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	200 mM ammonium sulfate, 25 % PEG 3350, 100 mM TRIS pH 8.5