9H35

Crystal structure of the YTHDC2 YTH domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-27
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.96863
Spacegroup name	P 61 2 2
Unit cell lengths	115.972, 115.972, 205.847
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.790 - 2.680
R-factor	0.2294
Rwork	0.227
R-free	0.28430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.986
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.800	2.860
High resolution limit [Å]	2.680	2.680
Number of reflections	43492	7664
<I/σ(I)>	10.75
Completeness [%]	100.0
Redundancy	10.71
CC(1/2)	0.999	0.585

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	50 mM ammonium sulfate, 5 mM BIS-TRIS pH 6.5, 30 % PEG 600