9H1F

Cofilin-1 in complex with high-affinity Sybody B12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-09
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.97623
Spacegroup name	P 4 21 2
Unit cell lengths	121.575, 121.575, 52.346
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.490 - 1.800
R-factor	0.2037
Rwork	0.203
R-free	0.22630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.010
Data reduction software	XDS (VERSION Jun 30, 2023)
Data scaling software	Aimless (0.7.15)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.490	1.864
High resolution limit [Å]	1.800	1.800
Rmerge	0.085	1.897
Number of reflections	36823	3644
<I/σ(I)>	21.79	1.36
Completeness [%]	99.6
Redundancy	26.6
CC(1/2)	1.000	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.1 M Citric acid pH 4.0 and 2.4 M Ammonium sulfate