9H13
Crystal structure of OXA-163 apoenzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-22 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 122.553, 122.553, 160.430 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.690 - 1.440 |
| R-factor | 0.1692 |
| Rwork | 0.168 |
| R-free | 0.18590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.790 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.690 | 1.490 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Rpim | 0.030 | 1.665 |
| Number of reflections | 127598 | 12442 |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 40.1 | 38.9 |
| CC(1/2) | 0.999 | 0.348 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 292 | 0.1 M Tris pH 9.0, 32% PEG 550 |
