9H0O
Fucosylated Lacto-N-biose binding protein from Bifidobacterium longum subsp. infantis in complex with Lacto-N-biose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 2.075 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.070, 52.560, 149.319 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.580 - 2.140 |
| R-factor | 0.2033 |
| Rwork | 0.202 |
| R-free | 0.23590 |
| Structure solution method | SAD |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.378 |
| Data reduction software | xia2 |
| Data scaling software | XDS |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.580 | 2.200 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Number of reflections | 21409 | 1112 |
| <I/σ(I)> | 10.35 | |
| Completeness [%] | 95.2 | |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2M NaCl, 0.1 M Tris pH 7.0, 30% PEG3000 + TCEP |
